It would be expected that syringe filters containing absolute rated membrane media would be approximately equivalent regardless of supplier for filters that have the same size, same membrane material and same pore size rating. This is not the case for syringe filters containing nominally rated media such as glass fiber or nonwoven polypropylene.
3802 36 Methods for Studying Methanogens and Methanogenesis in Marine Sediments. Allow 15 min for the iron and sulfide to react before checking the pH. If necessary, adjust. the pH to 7.2–7.4 w
Nov 19, 2017 · To prepare this cysteine hydrochloride stock solution, affix a sterile 0.2 μM filter (Whatman™, cat. number: 6780-2502) to a 5 mL syringe with Luer-Lok tip (BD™, cat. number: 309646) with the plunger removed. Add 3 ml ddH 2 O to the syringe and then add 0.75 g of cysteine hydrochloride (Sigma, cat. number: 52-89-1). Swirl the solution
Mar 01, 2001 · Cells were maintained at −80°C as a 30% (v/v) glycerol stock solution. For inoculum preparation, 1.8 ml of recombinant E. coli glycerol stock solution was added to 100 ml of medium with 10 g l −1 peptone, 5 g l −1 yeast extract, 5 g l −1 NaCl, 0.05 g l −1 chloramphenicol and 0.05 g l −1 ampicillin.
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Designed for scale-up suspension culture with space efficiency in mind, the EZBio® 5L Erlenmeyer flask is sterile and ready-to-use for shaker culture applications, and storage. High Density Polyethylene (HDPE) Cap with 0.2um hydrophobic filters for gas exchange without contamination.
The phosphorylation was positively correlated washed twice with 0.8 ml Tris-buffered saline (10 mM Tris–HCl, pH 8.3, 0.85% NaCl) that contained 0.5% (v/v) Triton X-100 and 0.02% with guard cell malate content and with stomatal aperture (w/v) SDS and subsequently twice more with only Tris-buffered sa- size.
Physical Characteristics: 13 mm diameter barrel 25mm diameter barrel Surface area (cm 2 ): 0.65 3.90 Sample volume (ml): <15 <105 Maximum pressure (bar): 45 90 Hold up volume (μl): 8.5 28.5 General Characteristics: • Disposable • Luer-Lok™ and pressure fitting • Ultrasonically bonded for reliability • Filter media type clearly inscribed on barrel • Excellent unit-to-unit and lot
Apr 30, 2019 · Fifty-milligram powder was weighed in a capped glass vial and then 1.5 mL of 2% HCl in methanol was added. Extracted solution was vortexed and sonicated for 15 min. Supernatant was collected and filtered through a 0.45μm PTFE syringe filter (VertiPure ™, Vertical Chromatography CO. LTD.). Each sample was analyzed by HPLC (Waters Associates
Durable, efficient, and broadly compatible, these pure, hydrophilic PTFE membranes are ideal for use with HPLC seperations, aqueous/organic solvents, and many of your other filtration needs. Pack of 100. Pore size: 0.50 microns. Diameter: 13 mm. Advantec P/N: H050A013A.
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A complete medium exchange was conducted daily via syringe ports. The chambers were then injected with 10 ml of incubator air through the sterile air filters on either end of the chamber. All rolled constructs had a matched control, which was grown under static conditions in a Petri dish rather than in
May 21, 2004 · N 2 O and CH 4 fluxes were measured by gas chromatography analyses of four syringe samples extracted from the same chambers at 10-min intervals [Verchot et al., 1999, 2000]. The PVC collars used for soil trace gas measurements were left in place throughout the course of the experiments.
Processes three to seven times more sample volume than filters without prefilter. Whatman™ GD/X Polypropylene Syringe Filters are specifically designed for filtration of viscous or otherwise hard-to-filter samples with high solids content.
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