The slurry was mixed intermittently every 20 min during the extraction. After 1 h, the samples were centrifuged at 21,952 rcf for 10 min, and the supernatant was filtered through a 0.45-mm PTFE syringe filter for the HPLC analysis with a Futecs model NS-4000 HPLC apparatus (Daejeon, Korea).
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