May 01, 2015 · Nutrient samples were filtered using a 0.45-μm sterile cartridge filter and kept frozen (− 80 °C) until processing on a Lachat flow-injection analyzer at the Institute of Marine Sciences Marine Analytical Lab at UCSC.
Nov 24, 2021 · Thereafter, it was centrifuged for 10 min at 8000 rpm and supernatant was filtered using Millipore filter of 0.45 μm pore size. The filtered extract was then subjected to UPLC-MS for identification.
Sub-samples were then passed through a 0.45 µL GF/F glass fiber syringe filter (Pall, Port Washington, New York, NY, USA) into sterile test tubes. Approximately 1.0 mL of each filtered sample was transferred into 1.5 mL scintillation vials and then stored at 4 °C for later conservative tracer analysis.
The supernatant was then freeze-dried, resuspended in distilled water, and filtered through 0.45 µm filter paper. The bacterial production of GABA was measured as follows. One milliliter of the culture prepared in Section 2.2 was transferred into 10 mL of MRS broth supplemented with 1% MSG and incubated at 37 °C for 48 h.
Dec 17, 2021 · In a sterile tissue culture hood, mix 5 mL FBS with 45 mL 50/50 DMEM/F12 media. We will refer to this solution as RPMI + 10% FBS. 33. Use a 0.22 μm syringe filter to sterilize the solution. 34. Store at 4°C for up to 2 months. a. Discard once the color of the media becomes closer to dark red/purple indicating the pH has increased.
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The hypodermic syringe, also known as the hypodermic needle, is a device used by medical professionals to transfer liquids into or out of the body. It is made up of a hollow needle, which is attached to a tube and a plunger.
Jul 17, 2017 · The production of IPV as described in Table 5.1 shows that the virus must be grown in cell culture, and then purified using three chromatography steps, followed by inactivation. As with any bioprocess, each steps results in loss of material that reduces the overall productivity.
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0.45 µm-pore filters allow water to pass but trap bacteria. 2. Filters are especially useful for samples with low numbers of bacteria since large volumes of fluid can be passed through. 3. Polycarbonate filters are better than the cellulose one because bacteria are caught on the filter surface more efficiently. 4.
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Mar 08, 2021 · For the cultivation in outdoor photobioreactors, seawater was chemically sterilized by using sodium hypochlorite. Active hypochlorite was deactivated by filtration over active carbon, followed by filtration across a filter with a pore size of 1 µm. Subsequently, the nutrient stock solution was added through a sterile filter (0.45 µm).
Dec 03, 2020 · Two materials of the syringe filter media were selected for the experiments: cellulose acetate (CA) and polyvinylidene fluoride (PVDF) (Acrodisc LC13 Syringe Filter, Pall Corporation, USA) with 13
Nov 20, 2018 · Culture broths of LAB were centrifuged at 8000 rpm (5810R, fixed angle type; Eppendorf, USA), serially diluted, and filtered through a 0.45-μm PTFE syringe filter (Whatman, USA). The content of organic acid was measured by HPLC (Waters Alliance e2695 system, USA) at 30°C using an Aminex HPX-87H column (300 mm × 7.8 mm, Bio-Rad, Hercules, CA
