The cell suspension was injected by a syringe pump and extruded through the mono air-jet device, under sterile conditions. The generated microbeads were hardened by an ionotropic gelling process into a 1.5% ( w/v ) BaCl 2 solution.
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Dec 17, 2021 · In a sterile tissue culture hood, mix 5 mL FBS with 45 mL 50/50 DMEM/F12 media. We will refer to this solution as RPMI + 10% FBS. 33. Use a 0.22 μm syringe filter to sterilize the solution. 34. Store at 4°C for up to 2 months. a. Discard once the color of the media becomes closer to dark red/purple indicating the pH has increased.
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The nanofibres are usually around 200–300 nm in diameter and have the typical mass per unit area less than 1 to 2 g/m 2, and some nanofibre filters could have as heavy as 15- to 90-g/m 2 stand-alone nanofiber web without the base substrate, 62 but the production cost is a concern.
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3.1.3 Mass Production. After the growth and sporulation processes have been thoroughly investigated and tested at laboratory level, mass production of the fungus can be undertaken on an industrial scale with various raw materials. Until now, entomopathogenic fungi have been produced in liquid or solid media. Production in Liquid Media
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Quality Production Laboratory Materials Facilities and Equipment Packaging and Labeling §21 CFR 211.110 - Sampling & testing of in-process materials & drug products - Key points
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